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The dehydrogenation of 1-(4-hydroxyphenyl)-ethanol to 4-hydroxyacetophenone represents the second reaction step during anaerobic degradation of p-ethylphenol in the denitrifying bacterium ‘Aromatoleum aromaticum' Eb N1.
Previous proteogenomic studies identified two different proteins (Chn A and Eb A309) as possible candidates for catalyzing this reaction [Wöhlbrand et al: J Bacteriol 2008;199-5709].
Also covered are two emerging expression systems, Methylobacterium extorquens AM1 and Caulobacter crescentus. Niebauer and Anne Skaja Robinson Chapter 6 Production of Recombinant Proteins Using the Methylotrophic Yeasts Pichia pastoris and Pichia methanolica Bruce L. Lesnicki and Sanjay Jain Chapter 7 Advances in Protein Expression in Filamentous Fungi Hendrik J. Kelley, and Michael Ward Chapter 8 Insect Cell Expression Systems Karthik Viswanathan, Yu-chan Chao, John T.
An essential reference, this book will enable both new and experienced expression technologists to make educated choices on the technology that is best suited for their needs. Kelly Chapter 2 Expression, Folding and Degradation in Escherichia coli Mirna Mujacic and François Baneyx Chapter 3 Tools for Metabolic Engineering in Escherichia coli Christina D.